Alkaline Phosphatase-X-biotin (1ml)
Alkaline Phosphatase-X-Biotin is an enzyme conjugate designed for use as an enzymatic marker in avidin-biotin-based immunoassays, including enzyme-linked immunosorbent assays (ELISA) and Western blotting. This conjugate consists of alkaline phosphatase (AP) covalently linked to biotin molecules, with approximately 12 to 14 molecules of biotin per alkaline phosphatase enzyme. The biotinylation provides strong binding affinity to avidin or streptavidin, facilitating specific detection in immunoassay systems. Alkaline phosphatase catalyzes the hydrolysis of substrates such as p-nitrophenyl phosphate to produce a measurable color change, allowing for the quantitative measurement of target proteins or antibodies.
Features:
Enzyme: Alkaline Phosphatase (AP)
Conjugate: Biotinylated alkaline phosphatase (12-14 biotin molecules per AP molecule)
Marker: Enzymatic activity detectable via colorimetric or chemiluminescent assays
Specificity: Strong binding to avidin or streptavidin for enhanced detection sensitivity
Applications: Immunoassays, ELISA, Western blotting, protein-protein interaction studies
Stability: Stable in a variety of assay conditions, providing consistent performance across multiple experiments
Applications:
Alkaline Phosphatase-X-Biotin is ideal for:
Avidin-Biotin Immunoassays: Detecting target molecules through avidin-biotin interaction in assays such as ELISA and Western blotting.
Enzyme-Linked Immunoassays (ELISA): Measuring antibodies or antigens with high sensitivity and specificity.
Protein-Protein Interaction Studies: Investigating the binding affinity and interactions of proteins or other biomolecules.
Diagnostic Assays: Detecting and quantifying biomarkers or analytes in clinical samples using a colorimetric or chemiluminescent readout.
Alkaline Phosphatase-X-Biotin is an enzyme conjugate designed for use as an enzymatic marker in avidin-biotin-based immunoassays, including enzyme-linked immunosorbent assays (ELISA) and Western blotting. This conjugate consists of alkaline phosphatase (AP) covalently linked to biotin molecules, with approximately 12 to 14 molecules of biotin per alkaline phosphatase enzyme. The biotinylation provides strong binding affinity to avidin or streptavidin, facilitating specific detection in immunoassay systems. Alkaline phosphatase catalyzes the hydrolysis of substrates such as p-nitrophenyl phosphate to produce a measurable color change, allowing for the quantitative measurement of target proteins or antibodies.
Features:
Enzyme: Alkaline Phosphatase (AP)
Conjugate: Biotinylated alkaline phosphatase (12-14 biotin molecules per AP molecule)
Marker: Enzymatic activity detectable via colorimetric or chemiluminescent assays
Specificity: Strong binding to avidin or streptavidin for enhanced detection sensitivity
Applications: Immunoassays, ELISA, Western blotting, protein-protein interaction studies
Stability: Stable in a variety of assay conditions, providing consistent performance across multiple experiments
Applications:
Alkaline Phosphatase-X-Biotin is ideal for:
Avidin-Biotin Immunoassays: Detecting target molecules through avidin-biotin interaction in assays such as ELISA and Western blotting.
Enzyme-Linked Immunoassays (ELISA): Measuring antibodies or antigens with high sensitivity and specificity.
Protein-Protein Interaction Studies: Investigating the binding affinity and interactions of proteins or other biomolecules.
Diagnostic Assays: Detecting and quantifying biomarkers or analytes in clinical samples using a colorimetric or chemiluminescent readout.
Alkaline Phosphatase-X-Biotin is an enzyme conjugate designed for use as an enzymatic marker in avidin-biotin-based immunoassays, including enzyme-linked immunosorbent assays (ELISA) and Western blotting. This conjugate consists of alkaline phosphatase (AP) covalently linked to biotin molecules, with approximately 12 to 14 molecules of biotin per alkaline phosphatase enzyme. The biotinylation provides strong binding affinity to avidin or streptavidin, facilitating specific detection in immunoassay systems. Alkaline phosphatase catalyzes the hydrolysis of substrates such as p-nitrophenyl phosphate to produce a measurable color change, allowing for the quantitative measurement of target proteins or antibodies.
Features:
Enzyme: Alkaline Phosphatase (AP)
Conjugate: Biotinylated alkaline phosphatase (12-14 biotin molecules per AP molecule)
Marker: Enzymatic activity detectable via colorimetric or chemiluminescent assays
Specificity: Strong binding to avidin or streptavidin for enhanced detection sensitivity
Applications: Immunoassays, ELISA, Western blotting, protein-protein interaction studies
Stability: Stable in a variety of assay conditions, providing consistent performance across multiple experiments
Applications:
Alkaline Phosphatase-X-Biotin is ideal for:
Avidin-Biotin Immunoassays: Detecting target molecules through avidin-biotin interaction in assays such as ELISA and Western blotting.
Enzyme-Linked Immunoassays (ELISA): Measuring antibodies or antigens with high sensitivity and specificity.
Protein-Protein Interaction Studies: Investigating the binding affinity and interactions of proteins or other biomolecules.
Diagnostic Assays: Detecting and quantifying biomarkers or analytes in clinical samples using a colorimetric or chemiluminescent readout.